4,6-Dichloroisatin is primarily used as a key intermediate in the synthesis of various pharmaceutical compounds. It plays a significant role in the development of drugs targeting neurological disorders, such as epilepsy and anxiety, due to its ability to modulate specific receptors in the brain. Additionally, it is utilized in the preparation of bioactive molecules with potential anticancer properties, as it can influence cell proliferation and apoptosis. The compound is also explored in the field of organic chemistry for the creation of novel heterocyclic compounds, which are valuable in drug discovery and material science. Its versatile reactivity makes it a valuable building block in medicinal chemistry research.