D-Talitol, a rare sugar alcohol (polyol), is primarily used as a high-purity reference standard in biochemical research and analytical chemistry, including techniques such as gas chromatography (GC), infrared spectrometry (IR), melting point determination, and polarimetry. It finds applications in the food industry as a low-calorie sweetener and sugar substitute suitable for diabetic-friendly and weight management products due to its ability to provide sweetness without high caloric content. In the pharmaceutical sector, it serves as an excipient in tablet formulations, aiding in the stabilization and delivery of active ingredients. Additionally, D-Talitol is utilized in cosmetic products as a humectant, helping to retain moisture and improve skin hydration. Its non-toxic and biocompatible nature makes it valuable in R&D for biochemical studies.