Monofucosyllacto-N-hexaose I is primarily utilized in research focused on understanding human milk oligosaccharides (HMOs) and their biological functions. It plays a significant role in studies investigating its prebiotic effects, as it promotes the growth of beneficial gut bacteria, particularly in infants. This compound is also explored for its potential to modulate the immune system, enhancing resistance to infections by acting as a decoy for pathogens. Additionally, it is used in the development of infant formula to mimic the composition and benefits of human breast milk. Researchers also study its role in cell signaling and its potential therapeutic applications in gastrointestinal and immune-related disorders.