Ammonium acetate is widely used in biochemical research as a volatile buffer in HPLC and LC-MS analyses, helping to maintain a stable pH during compound separation without leaving residues that could interfere with detection. It is also employed in protein purification, precipitation, and crystallization processes due to its compatibility with biological systems. In organic synthesis, it serves as a source of ammonia in various reactions, such as the preparation of acetamides and in the Ritter reaction. Additionally, it functions as an acidity regulator (E264) in food products for pH control and preservation. It is used in pharmaceutical manufacturing, dye production, and as a reagent in analytical chemistry.