BIX-01294 is primarily used in research settings as a potent and selective inhibitor of histone methyltransferases, particularly G9a and GLP. Its application is significant in the study of epigenetic regulation, as it helps to understand the role of histone methylation in gene expression and cellular processes. Researchers utilize BIX-01294 to investigate its effects on chromatin structure, DNA methylation, and cellular differentiation. It has also been explored in the context of cancer research, where inhibition of histone methyltransferases may offer potential therapeutic strategies. Additionally, BIX-01294 is employed in studies related to stem cell biology, where it aids in reprogramming and understanding the mechanisms of pluripotency. Its ability to modulate epigenetic marks makes it a valuable tool for probing the complex interplay between histone modifications and cellular function.