EHMT2-IN-2 is primarily used in research settings to study the role of EHMT2 (also known as G9a) in epigenetic regulation. It serves as a potent and selective inhibitor of EHMT2, an enzyme involved in the methylation of histone H3, which plays a critical role in gene expression and chromatin remodeling. Researchers utilize EHMT2-IN-2 to investigate its effects on cancer cell proliferation, as EHMT2 is often overexpressed in various cancers and contributes to tumor growth and metastasis. Additionally, this compound is employed in studies exploring its potential in reversing aberrant gene silencing, which could have implications for developing therapies for diseases linked to epigenetic dysregulation, such as neurological disorders and certain types of cancer. Its application extends to understanding cellular differentiation and the role of histone methylation in stem cell biology.