Used primarily in microbiology for staining biological specimens, it helps in identifying bacterial cells by highlighting their shapes and structures under a microscope. It is particularly effective in differentiating between live and dead cells, as it selectively stains dead cells blue. Additionally, it is employed in diagnostic procedures, such as the detection of Helicobacter pylori in gastric biopsies. The solution is also utilized in histology to enhance the visibility of specific tissue components. Its staining properties make it a valuable tool in educational and research laboratories for studying cellular morphology.