Methyl Violet 2B is widely used as a dye in the textile industry for coloring fabrics, particularly cotton, silk, and paper. It is also employed in biological staining to differentiate cell types under a microscope, especially in Gram staining to identify bacteria. In the printing industry, it serves as a colorant for inks and toners. Additionally, it finds application in the production of some types of pens and markers. Its use extends to the field of analytical chemistry as a pH indicator, where it changes color in response to varying acidity levels. Furthermore, it has antimicrobial properties and is used in some disinfectants and antiseptics for treating minor skin infections.