Widely used in histology laboratories for staining cell nuclei in tissue sections. Provides a clear, consistent blue to black nuclear stain that enhances contrast against cytoplasm and extracellular components. Commonly applied in routine diagnostic pathology and research settings, especially in biopsies and surgical specimens. Works effectively with both manual and automated staining protocols. Frequently used in combination with eosin in H&E staining, the most common histological staining method. Offers reliable differentiation and long-term stain retention, making it suitable for archival tissue samples. Compatible with various tissue types and fixation methods, including formalin-fixed paraffin-embedded tissues.