Acetocarmine solution is widely used in biological and cytogenetic studies as a nuclear stain. It is particularly effective for staining chromosomes, making it valuable in microscopy techniques to observe cell division processes such as mitosis and meiosis. The solution helps in visualizing the nucleus and chromosomal structures in plant and animal cells, especially during karyotype analysis. It is commonly used in squash preparations for examining root tips and other meristematic tissues. Due to its ability to bind to DNA, acetocarmine is also employed in assessing pollen viability and in some histological examinations. Its staining properties allow quick and clear identification of nuclear material under light microscopy.