Alcian Blue 8GX is widely used in histology and pathology for staining acidic polysaccharides, such as glycosaminoglycans, in tissues. It is particularly effective in identifying mucins in epithelial tissues, making it a valuable tool for diagnosing conditions like mucin-producing tumors. The dye is also employed in research to study extracellular matrix components, cartilage, and connective tissues. Its ability to selectively bind to sulfated and carboxylated glycoproteins makes it essential for visualizing structures in biological samples under a microscope. Additionally, it is used in combination with other stains to enhance contrast and specificity in tissue analysis.