Azocarmine G is primarily used as a biological stain in histology and microscopy. It is particularly effective in staining acidic tissue components, such as cytoplasm and collagen, making it a valuable tool for differentiating cellular structures under a microscope. The dye is often employed in combination with other stains to enhance contrast and provide clearer visualization of tissue samples. Additionally, Azocarmine G is utilized in some specialized staining techniques to study specific tissue types or pathological conditions, aiding in diagnostic and research applications. Its vibrant color and staining properties make it a reliable choice for detailed microscopic analysis in biological and medical studies.