Chromotrope 2R is widely used as a biological stain in histology and microscopy. It is particularly effective for staining connective tissues, collagen fibers, and mucopolysaccharides, enabling clear visualization under a microscope. In medical diagnostics, it aids in identifying abnormal tissue structures, making it valuable for pathological studies. Additionally, it is employed in textile industries as an acid dye for coloring wool, silk, and nylon, providing vibrant and durable shades. Its application extends to pH indicators in some analytical procedures, where it helps determine acidity or alkalinity based on color changes.