6-Chloroflavone is used primarily in pharmaceutical research due to its biological activity. It serves as a key intermediate in the synthesis of flavonoid-based drugs, which exhibit anti-inflammatory, antioxidant, and anticancer properties. This compound is also investigated for its potential to modulate enzyme activity, particularly in relation to cytochrome P450 enzymes, making it valuable in drug metabolism studies. Additionally, 6-Chloroflavone has shown promise in neuroprotective research, where it may help in developing treatments for neurodegenerative disorders. Its ability to interact with cellular signaling pathways further supports its use in biochemical assays and therapeutic development.