PF-5274857 is primarily utilized in research settings, particularly in the field of oncology. It functions as a selective inhibitor of the Hedgehog signaling pathway, which plays a crucial role in cell proliferation and differentiation. By targeting this pathway, it helps in studying the mechanisms of various cancers, especially those driven by abnormal Hedgehog signaling, such as basal cell carcinoma and medulloblastoma. Researchers use it to explore potential therapeutic strategies and to understand the molecular basis of tumor growth and progression. Additionally, it serves as a valuable tool in preclinical studies to evaluate the efficacy of new cancer treatments.