Used as a key intermediate in the synthesis of biologically active compounds, particularly HDAC inhibitors for research in cancer therapy and neurodegenerative diseases. The N'-hydroxy carboximidamide functionality allows for further derivatization to modulate protein acetylation, promoting cell cycle arrest and apoptosis in tumor cells. Valuable in preclinical studies for epigenetic regulation, immune modulation, inflammation, autoimmune disorders, and viral latency by enabling alteration of gene expression. The 5-trifluoromethyl group enhances cell membrane permeability and molecular stability, improving binding to biological targets. Commonly employed in high-throughput screening for hit identification in drug discovery projects. Its structure facilitates strong interactions with zinc-containing enzymes.