5-fluoro-indirubin is primarily utilized in biomedical research due to its potent inhibitory effects on specific kinases, particularly glycogen synthase kinase-3β (GSK-3β). This property makes it a valuable compound in studying cellular signaling pathways, especially those involved in diseases like cancer, neurodegenerative disorders, and diabetes. Its ability to modulate cell cycle progression and apoptosis has led to its use in exploring potential therapeutic strategies for these conditions. Additionally, it serves as a chemical tool in molecular biology to investigate the role of GSK-3β in various biological processes, including stem cell differentiation and embryonic development.