Trehalase, sourced from porcine kidney, is primarily utilized in biochemical research and industrial applications. It plays a crucial role in the hydrolysis of trehalose into glucose, making it essential in studies involving carbohydrate metabolism and energy regulation. In the food industry, it is employed to modify trehalose levels in products, enhancing texture, shelf life, and sweetness. Additionally, it is used in diagnostic kits to measure trehalase activity, aiding in the detection of metabolic disorders. Its application in biotechnology includes the production of glucose from trehalose in fermentation processes, supporting the development of biofuels and other bioproducts.