β-N-Acetylglucosaminidase from Canavalia ensiformis is widely used in biochemical research for the hydrolysis of N-acetylglucosamine residues in glycoproteins, glycolipids, and oligosaccharides. It is particularly valuable in studying the structure and function of complex carbohydrates, aiding in the analysis of glycosylation patterns in proteins. This enzyme is also employed in the preparation of glycans for mass spectrometry and chromatography, enhancing the accuracy of glycomics studies. Additionally, it finds application in the food industry for modifying polysaccharides to improve texture and stability. In medical diagnostics, it is utilized to detect specific glycosidase activities, which can be indicative of certain metabolic disorders. Its role in biotechnology includes the synthesis of bioactive compounds and the modification of natural products for enhanced efficacy.