Chitotriose trihydrochloride hydrate is widely used in biochemical research as a substrate or inhibitor to study the activity of chitinases and other enzymes involved in chitin metabolism. It serves as a valuable tool for investigating the mechanisms of chitin degradation, which is crucial for understanding fungal infections and developing antifungal therapies. Additionally, it is utilized in the study of plant-pathogen interactions, as chitin is a key component of fungal cell walls. This compound also finds application in the development of chitin-based biomaterials and in research focused on immune system modulation, as chitin derivatives can influence immune responses. Its role in enzymatic assays and structural studies further highlights its importance in advancing scientific knowledge in biochemistry and molecular biology.