Used in microbiology laboratories for the cultivation of fastidious microorganisms, particularly those found in clinical specimens such as blood, wound exudates, and body fluids. Supports the growth of a wide range of bacteria, including streptococci, staphylococci, and anaerobes. Commonly used for isolation and identification of pathogenic organisms in blood cultures and for hemolysis studies due to its ability to support clear hemolytic reactions. Often supplemented with blood (typically sheep or horse) to enhance growth and detect hemolytic patterns. Widely applied in diagnostic settings for detecting infections like strep throat, bacterial endocarditis, and other invasive diseases.