Gap 26 is widely used in research to study the role of connexin-based gap junctions in cellular communication. It is particularly applied in experiments to inhibit connexin 43 (Cx43) and connexin 37 (Cx37) channels, which are crucial in various physiological processes. Researchers use Gap 26 to investigate its effects on cell signaling, especially in cardiovascular studies, where it helps understand the regulation of heart function and vascular responses. Additionally, it is employed in neuroscience to explore the impact of gap junction inhibition on neuronal activity and brain function. Gap 26 is also utilized in studies related to wound healing, inflammation, and cancer, as gap junctions play a role in these processes. Its application provides insights into potential therapeutic targets for diseases involving dysregulated cell communication.