Pteridin-4-ol is primarily used as a key intermediate in the synthesis of biologically active compounds, especially in pharmaceuticals and research chemicals. It plays a significant role in the development of inhibitors targeting enzymes involved in metabolic pathways, such as dihydrofolate reductase (DHFR), which is crucial in cancer and antimicrobial drug design. Due to its structural similarity to pteridine derivatives found in coenzymes like tetrahydrobiopterin, it is also employed in biochemical studies to understand redox processes and enzyme mechanisms. Additionally, Pteridin-4-ol serves as a building block in the preparation of fluorescent probes and sensors, leveraging its photophysical properties for detecting metal ions or biomolecules in analytical and diagnostic applications. Its utility in medicinal chemistry and life sciences research makes it valuable for drug discovery and biochemical tool development.