Polyoxin B is widely used in agriculture as a fungicide, particularly effective against fungal pathogens that affect crops. It is applied to protect fruits, vegetables, and ornamental plants from diseases such as powdery mildew, leaf spot, and blight. The compound works by inhibiting the synthesis of chitin, a crucial component of fungal cell walls, thereby preventing the growth and spread of the fungi. Its targeted mode of action makes it an environmentally friendly option, as it minimizes harm to non-target organisms. Additionally, Polyoxin B is often integrated into integrated pest management (IPM) programs due to its low toxicity and compatibility with beneficial insects. It is also used in post-harvest treatments to extend the shelf life of produce by controlling fungal infections during storage.