Isosteviol is widely used in the food and beverage industry as a natural sweetener due to its high sweetness intensity and low-calorie content. It serves as a sugar substitute in products like soft drinks, desserts, and confectionery, catering to health-conscious consumers and those managing diabetes. In the pharmaceutical sector, it is explored for its potential therapeutic properties, including anti-inflammatory, antihypertensive, and antioxidant effects, making it a candidate for developing treatments for cardiovascular diseases and metabolic disorders. Additionally, isosteviol is utilized in cosmetic formulations for its skin-soothing and anti-aging properties, enhancing the appeal of skincare products. Its versatility and natural origin make it a valuable ingredient across multiple industries.