Fumonisin B2 is primarily associated with contamination in agricultural products, especially corn and corn-based foods. It is a mycotoxin produced by fungi of the genus Fusarium, and its presence poses significant concerns in food and feed safety. The main application context of Fumonisin B2 is in toxicological research and food safety monitoring due to its harmful effects on human and animal health. It is studied extensively for its role in causing liver and kidney toxicity and is classified as a possible human carcinogen. Regulatory agencies use detection of Fumonisin B2 as a marker to assess the safety of grain supplies. It is also used in research models to understand the mechanisms of sphingolipid disruption, as it inhibits ceramide synthase, leading to accumulation of sphinganine and related metabolites. Due to its toxicity, Fumonisin B2 has no commercial or industrial application as a useful chemical. Instead, its relevance lies in monitoring and controlling exposure in the food chain, ensuring compliance with safety standards, and supporting studies on mycotoxin detoxification methods.