Hoechst 33258 analog is widely utilized in biological research for its ability to bind specifically to DNA, particularly in the minor groove of AT-rich sequences. This property makes it an essential tool in fluorescence microscopy and flow cytometry for staining and visualizing DNA in live or fixed cells. It is commonly employed in cell cycle analysis to distinguish between different phases based on DNA content. Additionally, it is used in chromosome and nuclear staining to study cellular structures and processes. Its fluorescence properties also make it suitable for detecting DNA in gel electrophoresis and other molecular biology techniques. In drug discovery, it serves as a probe to study DNA interactions with potential therapeutic compounds.