Nile Red is widely used as a fluorescent dye in biological and chemical research. It is particularly effective in staining and detecting lipid droplets in cells, making it a valuable tool in studying lipid metabolism and storage. The dye’s ability to fluoresce in hydrophobic environments allows researchers to visualize and analyze lipid distribution and accumulation in various cell types. Additionally, Nile Red is employed in environmental studies to detect and quantify microplastics in water samples due to its affinity for hydrophobic surfaces. Its fluorescence properties also make it useful in material science for characterizing polymers and nanoparticles. Overall, Nile Red serves as a versatile marker in both biological and environmental applications.