Used extensively as a fluorescent tracer in biochemical and medical research, this compound aids in tracking and visualizing biological processes. Its high fluorescence intensity makes it suitable for labeling biomolecules, enabling detailed study of cellular interactions and dynamics. It is also employed in flow cytometry and fluorescence microscopy to detect and analyze specific cells or molecules. Additionally, it serves as a pH-sensitive probe in various assays, helping to measure pH changes in biological systems. Its stability and sensitivity make it a valuable tool in diagnostics and experimental studies.