Ani 9 is primarily used as a fluorescent probe in biochemical and cellular imaging applications. It exhibits strong fluorescence in hydrophobic environments, making it particularly useful for studying protein folding, membrane dynamics, and amyloid formation. Researchers employ Ani 9 to detect and monitor conformational changes in proteins, especially in the context of neurodegenerative diseases where protein aggregation plays a key role. Its high sensitivity to environmental polarity allows for real-time tracking of molecular interactions in live cells and in vitro systems. Additionally, Ani 9 is utilized in high-throughput screening assays to identify compounds that modulate protein aggregation, supporting drug discovery efforts targeting conditions like Alzheimer's and Parkinson’s disease.