Used as a topical antiseptic for minor wounds and skin infections due to its antimicrobial properties. Commonly applied in veterinary and aquaculture settings to treat bacterial and protozoal infections in fish. Also utilized in biological research as a fluorescent stain for microscopy and as a nucleic acid binding agent. Has been studied for antiviral activity, including against some enveloped viruses. Occasionally used in cell culture to eliminate mycoplasma contamination.