5(6)-Aminofluorescein is widely used as a fluorescent tracer in biological and chemical research. Its strong fluorescence makes it ideal for labeling and tracking molecules in various assays, such as cell imaging and flow cytometry. It is also employed in the study of enzyme activity, where it can act as a substrate or probe to monitor biochemical reactions. Additionally, it is utilized in environmental monitoring to trace water flow and contamination due to its high detectability under UV light. Its applications extend to diagnostics, where it aids in the detection of specific biomolecules in clinical samples.