Used extensively in biological and chemical research as a fluorescent tracer and pH indicator. It helps in tracking cellular processes and measuring intracellular pH levels due to its strong fluorescence properties. Commonly applied in flow cytometry, fluorescence microscopy, and molecular biology assays to visualize and quantify biological molecules. Its high sensitivity makes it valuable in detecting enzymatic activities and studying cell viability. Additionally, it is utilized in environmental monitoring to trace pollutants and assess water quality.