Isoalloxazine is a key component in the structure of flavin molecules, such as flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), which are crucial cofactors in various enzymatic reactions. It plays a vital role in redox reactions, facilitating electron transfer in metabolic pathways like cellular respiration and photosynthesis. Isoalloxazine is also utilized in the development of fluorescent dyes and probes for biochemical research, enabling the study of cellular processes and molecular interactions. Additionally, its derivatives are explored in pharmaceutical research for potential therapeutic applications, particularly in targeting oxidative stress-related diseases.