Pristanic acid solution is commonly used in biochemical research, particularly in studies related to peroxisomal metabolism and fatty acid oxidation disorders. It serves as a substrate to assess the activity of enzymes involved in the alpha-oxidation pathway, which is critical for the breakdown of branched-chain fatty acids. Due to its structural similarity to endogenous fatty acids, it is also utilized in cell culture experiments to investigate metabolic responses and mitochondrial function. In clinical diagnostics, pristanic acid solution acts as a reference standard in gas chromatography-mass spectrometry (GC-MS) analyses to detect abnormal accumulations in patients suspected of having peroxisomal diseases, such as Refsum disease or Zellweger syndrome. Its controlled application helps in evaluating metabolic profiles and diagnosing inborn errors of metabolism. Additionally, it is employed in lipidomics research to understand the role of branched-chain fatty acids in cellular signaling and energy homeostasis. Due to its solubility characteristics, the solution form allows for precise dosing and ease of handling in experimental assays.