Choloylglycine Hydrolase, derived from Clostridium perfringens, is primarily used in biochemical research and diagnostic applications. It plays a key role in the hydrolysis of bile acid conjugates, such as cholylglycine, into free bile acids and glycine. This enzymatic activity is particularly useful in studying bile acid metabolism and its impact on digestive processes. In clinical diagnostics, it is employed to measure bile acid levels in serum and other biological samples, aiding in the assessment of liver function and the diagnosis of hepatobiliary disorders. Additionally, it is utilized in the development of enzyme-based assays to investigate the gut microbiome's role in bile acid transformation, contributing to research on metabolic diseases and gut health.