Carnitine Acetyltransferase from pigeon breast muscle is widely used in biochemical research to study metabolic processes, particularly those involving fatty acid metabolism. It plays a critical role in catalyzing the transfer of acetyl groups between coenzyme A and carnitine, a process essential for the transport of fatty acids into mitochondria for energy production. This enzyme is utilized in assays to measure carnitine levels and to investigate mitochondrial function, making it valuable in studies related to metabolic disorders, such as fatty acid oxidation defects. Additionally, it aids in understanding the regulation of energy metabolism in various tissues, contributing to advancements in nutritional and physiological research.