Phospholipase C from Bacillus cereus is widely used in biochemical research to study cell signaling pathways. It plays a crucial role in hydrolyzing phosphatidylinositol 4,5-bisphosphate (PIP2) into diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), which are important secondary messengers in cellular processes. This enzyme is utilized in experiments to investigate the regulation of calcium release and protein kinase C activation in cells. Additionally, it is employed in industrial applications, such as the modification of phospholipids in food and pharmaceutical products to improve their functional properties. Its ability to cleave specific phospholipid bonds makes it valuable in the production of emulsifiers and other lipid-based ingredients.