Enolase, derived from baker's yeast (S. cerevisiae), is widely utilized in biochemical research and industrial applications due to its role in glycolysis. It is employed as a key enzyme in metabolic studies to understand cellular energy production and glucose metabolism. In diagnostics, enolase serves as a biomarker for certain diseases, including cancer, where its levels are often elevated. Additionally, it is used in enzyme assays and as a reference protein in laboratory experiments to study enzyme kinetics and protein interactions. Its stability and specificity make it valuable in biotechnological processes, such as the production of biofuels and other biochemical products.