Aconitase from porcine heart is widely utilized in biochemical research to study the tricarboxylic acid (TCA) cycle, also known as the Krebs cycle. It plays a critical role in catalyzing the conversion of citrate to isocitrate, a key step in cellular energy production. Researchers employ this enzyme to investigate metabolic pathways, enzyme kinetics, and mitochondrial function. It is also used in studies related to iron-sulfur cluster proteins, as aconitase is known to contain an iron-sulfur cluster essential for its activity. Additionally, it serves as a tool in understanding the impact of oxidative stress on enzyme activity, as aconitase is highly sensitive to reactive oxygen species. Its application extends to the development of assays for diagnosing metabolic disorders and evaluating the efficacy of potential therapeutic agents targeting mitochondrial dysfunction.