Phosphoriboisomerase from spinach is primarily utilized in biochemical research and enzymatic studies due to its role in the pentose phosphate pathway. It catalyzes the interconversion of ribose-5-phosphate and ribulose-5-phosphate, making it valuable for investigating metabolic pathways and enzyme kinetics. This enzyme is also employed in the synthesis of nucleotides and other biomolecules, aiding in the study of cellular metabolism and energy production. Additionally, it serves as a tool in genetic engineering and biotechnology for manipulating sugar metabolism in various organisms. Its application extends to educational laboratories, where it is used to demonstrate enzyme activity and metabolic processes to students.