3-phosphoglyceric phosphokinase plays a crucial role in glycolysis, where it catalyzes the transfer of a phosphate group from 1,3-bisphosphoglycerate to ADP, forming ATP and 3-phosphoglycerate. This reaction is essential for energy production in cells, as it generates ATP, the primary energy currency of the cell. In metabolic studies, this enzyme is often used to understand energy metabolism and cellular respiration. Additionally, it is significant in biotechnology and biochemistry research, particularly in the development of metabolic engineering strategies to optimize energy production in microorganisms. Its function is also studied in the context of diseases related to metabolic dysregulation, such as cancer, where altered glycolysis pathways are a key feature.