Cholesterol esterase from porcine pancreas is widely used in clinical diagnostics, particularly in the measurement of cholesterol levels in blood samples. It plays a critical role in enzymatic assays where it hydrolyzes cholesterol esters into free cholesterol and fatty acids, enabling accurate quantification of total cholesterol. This enzyme is also utilized in research studies focused on lipid metabolism, atherosclerosis, and cardiovascular diseases, as it helps in understanding the mechanisms of cholesterol absorption and esterification. Additionally, it finds application in the food industry for analyzing cholesterol content in various food products, ensuring compliance with nutritional labeling regulations. Its specificity and efficiency make it a valuable tool in both biomedical and industrial settings.