Phospholipase A2 from Crotalus adamanteus venom is widely used in biochemical research to study lipid metabolism and membrane dynamics. It plays a critical role in hydrolyzing phospholipids at the sn-2 position, releasing fatty acids and lysophospholipids. This enzyme is utilized in investigating inflammatory pathways, as it is involved in the production of arachidonic acid, a precursor for pro-inflammatory mediators. Additionally, it is employed in studying cell signaling mechanisms and membrane remodeling processes. In pharmaceutical research, it serves as a tool for developing inhibitors targeting phospholipase A2 activity, which could have therapeutic potential in treating inflammatory diseases. Its specificity and activity make it valuable for analyzing lipid-protein interactions and understanding the structural and functional properties of biological membranes.