Cellulose Azure is widely used as a substrate in biochemical and microbiological research to study the activity of cellulase enzymes. It serves as a colorimetric indicator, allowing researchers to measure and quantify cellulase activity by observing the release of soluble blue dye when the enzyme breaks down the cellulose. This application is particularly valuable in industries such as biofuel production, where understanding and optimizing cellulose degradation is crucial for efficient biomass conversion. Additionally, it is utilized in environmental studies to assess the degradation of cellulose-based materials in natural ecosystems. Its ease of use and visual detection make it a practical tool for laboratory experiments and enzyme assays.