Used as a reagent in organic synthesis, particularly in phosphorylation reactions where selective protection and deprotection of hydroxyl groups are required. Its nitrophenyl group acts as a chromophore, enabling easy monitoring of reaction progress by UV spectroscopy. Commonly employed in the preparation of phosphate esters and nucleotide analogs for biochemical and pharmaceutical research. Also utilized in enzyme inhibition studies due to its structural similarity to phosphate-containing substrates.