Used extensively in enzymatic assays, particularly for studying protease activity. Its primary application lies in fluorescence-based assays where the AMC (7-amino-4-methylcoumarin) group acts as a fluorescent reporter. When the compound is cleaved by a specific protease, free AMC is released, producing a measurable fluorescent signal. This makes it ideal for monitoring enzyme kinetics and inhibition in research related to proteolytic enzymes such as lysosomal proteases or caspases. The Boc and Tfa protecting groups enhance stability and substrate specificity, allowing selective detection in complex biological samples. Commonly used in high-throughput screening for drug discovery and biochemical studies.