FEN1-IN-3 is primarily used in research settings to study the inhibition of the Flap Endonuclease 1 (FEN1) enzyme, which plays a critical role in DNA replication and repair. By inhibiting FEN1, researchers can investigate its role in maintaining genomic stability and its potential as a target in cancer therapy. This compound is particularly valuable in understanding how disrupting DNA repair mechanisms can selectively target cancer cells, which often rely heavily on these pathways for survival. Additionally, FEN1-IN-3 aids in exploring the broader implications of FEN1 inhibition in diseases characterized by defective DNA repair processes. Its application is largely confined to laboratory studies aimed at developing novel therapeutic strategies.