Zebularine is primarily utilized in epigenetic research due to its ability to inhibit DNA methyltransferases, enzymes responsible for adding methyl groups to DNA. This inhibition leads to the reactivation of genes that have been silenced by methylation, making it a valuable tool in studying gene expression and epigenetic regulation. In cancer research, Zebularine is investigated for its potential to reverse aberrant DNA methylation patterns, which are often associated with tumor suppressor gene silencing. By demethylating and reactivating these genes, it may help restore normal cellular function and inhibit tumor growth. Additionally, Zebularine is explored in the development of epigenetic therapies for various diseases, including neurological disorders and autoimmune conditions, where abnormal DNA methylation plays a role. Its application extends to agricultural research, where it is used to study epigenetic modifications in plants, potentially improving crop traits and stress resistance.