Questin is used in research settings primarily as a biochemical tool for studying enzyme activity, particularly in investigations involving oxidoreductases and other metabolic enzymes. It serves as a substrate or inhibitor in assays designed to understand cellular respiration and electron transport mechanisms. Due to its structural properties, it also finds use in the development of fluorescent probes for detecting reactive oxygen species in biological systems. Additionally, Questin has been explored in antimicrobial studies for its potential to interfere with microbial electron transfer chains, showing activity against certain bacterial and fungal strains. Its role in laboratory research supports drug discovery and the design of novel therapeutic agents targeting metabolic pathways.